低密度脂蛋白受體(LDLR)重組蛋白供應-信帆生物

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10µg

Recombinant Low Density Lipoprotein Receptor (LDLR)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

12th Edition (Revised in Aug, 2016)[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E. coli

Residues: Thr497~Thr742

Tags: N-terminal His-Tag

Tissue Specificity: Liver, Brain, Placenta.

Subcellular Location: Cell membrane; Single-pass type I membrane protein.

Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.7

Predicted Molecular Mass: 30.9kDa

Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of

0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37oC for 48h, and no obvious degradation and precipitation were

observed. The loss rate is less than 5% within the expiration date under

appropriate storage condition.[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE

低密度脂蛋白受體(LDLR)重組蛋白供應-信帆生物